Cytochemical differentiation of the axon membrane in A- and C-fibres.
نویسندگان
چکیده
Guinea pig and rat sciatic nerves were fixed with cacodylate-buffered aldehydes and OsO4, and were stained with ferric ion and ferrocyanide. Cytoplasmic surfaces of the non-myelinated nodal axon membrane of A-fibres display distinct electron-dense aggregates of stain. These aggregates were not observed in association with the paranodal or internodal axolemma. The membranes of C-fibres exhibit no staining under these conditions. Thus, the nodal axolemma of normal myelinated fibres is structurally distinct from both the myelinated internodal membrane, and from the axolemma of C-fibres. The ferric ion-ferrocyanide technique may provide a method for marking axonal membrane with normal nodal properties.
منابع مشابه
Differentiation of Mouse Stem Cells into Neural Cells on PLGA Microspheres Scaffold
The cellular therapy and nerve tissue engineering will probably become a major therapeutic strategy for promoting axonal growth through injured area in central nervous system and peripheral nervous system in the coming years. The stem cell carrier scaffolds in nerve tissue engineering resulted in strong survival of cells and suitable differentiation into n...
متن کاملOptimization of Hardness Strengths Response of Plantain Fibres Reinforced Polyester Matrix Composites (PFRP) Applying Taguchi Robust Design
Volume fraction of fibres (A), aspect ratio of fibres (B) and fibres orientation (C) are considered as control factors in the determination of hardness strength, hardness strength of plantain fiber reinforced polyester composites (PFR P). These properties were determined for plantain empty fruit bunch (PEFB) and plantain pseudo stem (PPS). Hardness tests were conducted on the replicated samples...
متن کاملEffect of Mouse Liver Extract on in Vitro Differentiation of Amniotic Membrane Stem Cells into Hepatocyte-Like Cells
ABSTRACT Background and Objective: Multipotent placental amniotic membrane mesenchymal stem cells (MSCs) are capable of differentiating into specialized tissues under different conditions. The aim of this study was to induce differentiation of placental amniotic membrane MSCs from NMRI mouse into hepatocytes using liver extract. &nb...
متن کاملUltrastructural and cytochemical features of mammalian skeletal muscle fibres following denervation.
After denervation, the usual cytochemical criteria for identifying fibre types in mammalian skeletal muscle are lost, and thus ultrastructural analysis becomes essential. The width of the Z-line is a valuable criterion for the identification of fibre types in normal muscle, and the differences in width remain apparent even after denervation. In the red portion of the normal rat semitendinosus m...
متن کاملP-74: Cytochemical Evaluation of Sperm Chromatin and DNA Integrity in Couples with 3 Unexplained Recurrent Spontaneous Abortion
Background: The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. Materials and Methods: In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and cont...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of neurology, neurosurgery, and psychiatry
دوره 40 4 شماره
صفحات -
تاریخ انتشار 1977